Title: Naturally Complex: Botanical Dietary Supplement Safety Research at the National Toxicology Program
Speaker: Cynthia Rider, PhD, DABT Division of the National Toxicology Program National institute of Environmental Health Sciences(NIEHS) Research Triangle Park, NC
Date: Wednesday, October 18, 2017
Location: CAM Building, 17 East 102nd Street West Tower Elevator, 5th Floor, D5-122
Short Bio: Dr. Rider is a toxicologist with the National Toxicology Program (NTP), National Institute of Environmental Health Sciences (NIEHS), where she serves as project leader for a diverse portfolio of testing programs including polycyclic aromatic compounds, botanical dietary supplements (e.g., Ginkgo biloba extract, Garcinia cambogia), and industrial chemicals. Dr. Rider’s research interests are in evaluating and refining methods to predict mixture toxicity based on data from components or whole reference mixtures.
Abstract: The National Toxicology Program (NTP) has evaluated the toxicity and carcinogenicity of a number of widely-used botanical dietary supplements. Key challenges encountered in this research include: determining how diverse products in the marketplace relate to a single reference sample that has been chemically and toxicologically characterized (i.e., determining “sufficient similarity”), identifying the active constituent within the complex mixture, and understanding absorption, distribution, metabolism, and elimination (ADME) properties of botanicals. Current NTP research has been aimed at addressing these challenges. Case studies with Ginkgo biloba extract, black cohosh extract, and Echinacea purpurea extract were developed to evaluate approaches for assessing sufficient similarity. In each case, multiple extracts of each botanical were evaluated for chemical similarity using comparison of chromatographic profiles (untargeted approach) and quantification of known marker compounds (targeted approach). Biological similarity of the extracts was evaluated using both in vitro liver models and short term rodent genomic studies. Statistical and data visualization methods guided by expert judgement were then used to make decisions about similarity of extracts to the reference sample. These data were also used to identify potential active constituents within the mixtures. Along with statistical evaluation of high content chemical and biological data, bioassay-guided fractionation is being used to identify possible actives in black cohosh extract. Finally, ADME data generated for Ginkgo biloba extract in rodents will be compared to available human data to understand how animal doses relate to human exposure. This work will inform future research on botanical dietary supplements and application of data in safety assessments of these products.
If you need more information please contact:
*Light lunch will be provided
Wednesday, October 18 at 12:00pm to 1:00pm
CAM Buiduilding, 17 East 102nd, West Tower Elevator - 5th Floor, D5-122